We developed CaliAli, a comprehensive suite designed to extract neuronal signals from one-photon calcium imaging data collected across multiple sessions in free-moving conditions in mice. CaliAli ...

In-vivo imaging of the neuronal activity in mouse primary visual cortex. Left, high-resolution neuronal map; middle, high-speed neuronal activity recording captured by the two-photon microscope with ...

A growing body of researchers is utilizing optical imaging to monitor activity in the brain. One type of optical imaging, two-photon calcium imaging, has been widely adopted for its ability to record ...

Volumetric functional imaging is widely used for recording neuron activities in vivo, but there exist tradeoffs between the quality of the extracted calcium traces, imaging speed, and laser power.

Time-lapse two-photon imaging of the calcium indicator, GCaMP6f, in an acute slice of a 11-day-old mouse olfactory bulb (depth, 150 μm). GCaMP6f was expressed in mitral cells. The medium was exchanged ...

New ultra-fast sensors developed at Janelia can detect calcium ions nearly as fast as they are released from neurons, allowing scientists to tease out the individual, milliseconds-long signals passing ...